Integrated Microfluidic CustomArray Device for Bacterial Genotyping and Identification
| Autor: | Andy McShea, Robin Hui Liu, Michael Bizak, David Danley, Sandra B. Munro, Tony Siuda, Tai Nguyen, Dominic Suciu, Mike Slota, H. Sho Fuji |
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| Rok vydání: | 2006 |
| Předmět: |
010302 applied physics
DNA Microarray Chip biology Computer science Microfluidics 02 engineering and technology Computational biology 021001 nanoscience & nanotechnology biology.organism_classification Chip 01 natural sciences Molecular biology Computer Science Applications Bacillus anthracis Medical Laboratory Technology 0103 physical sciences Silicon chip Microarray hybridization DNA microarray 0210 nano-technology Genotyping |
| Zdroj: | JALA: Journal of the Association for Laboratory Automation. 11:360-367 |
| ISSN: | 1535-5535 |
| Popis: | The ongoing threat of the potential use of biothreat agents (such as Bacillus anthracis) as a biochemical weapon emphasizes the need for a rapid, miniature, fully automated, and highly specific detection assay. An integrated and self-contained microfluidic device has been developed to rapidly detect B. anthracis and many other bacteria. The device consists of a semiconductor-based DNA microarray chip with 12,000 features and a microfluidic cartridge that automates the fluid handling steps required to carry out a genotyping assay for pathogen identification. This fully integrated and disposable device consists of low-cost microfluidic pumps, mixers, valves, fluid channels, reagent storage chambers, and DNA microarray silicon chip. Microarray hybridization and subsequent fluid handling and reactions were performed in this fully automated and miniature device before fluorescent image scanning of the microarray chip. The genotyping results showed that the device was able to identify and distinguish B. anthracis from the other members of the closely related Bacillus cereus group, demonstrating the potential of integrated microfluidic and microarray technology for highly specific pathogen detection. The device provides a cost-effective solution to eliminate labor-intensive and time-consuming fluid handling steps and allows the detection and identification of biological warfare agents in a rapid and automated fashion. |
| Databáze: | OpenAIRE |
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