Flavonoids from Siparuna cristata as Potential Inhibitors of SARS-CoV-2 Replication

Autor: Amanda R. Tucci, Fernanda das Neves Costa, Rosineide Costa Simas, Pedro Henrique Nascimento, Romain Sausset, Maria Eduarda S Monteiro, Simony C. Mendonça, Manuela Leal da Silva, Caio Cheohen, Tayssa Santos Gondim, Carla Monteiro Leal, Natalia Fintelman-Rodrigues, Milene D. Miranda, Maria Eduarda A Esteves, Marilda M. Siqueira, Gilda Guimarães Leitão, Suzana G. Leitão
Rok vydání: 2021
Předmět:
Zdroj: Revista Brasileira de Farmacognosia. 31:658-666
ISSN: 1981-528X
DOI: 10.1007/s43450-021-00162-5
Popis: The novel coronavirus SARS-CoV-2 has been affecting the world, causing severe pneumonia and acute respiratory syndrome, leading people to death. Therefore, the search for anti-SARS-CoV-2 compounds is pivotal for public health. Natural products may present sources of bioactive compounds; among them, flavonoids are known in literature for their antiviral activity. Siparuna species are used in Brazilian folk medicine for the treatment of colds and flu. This work describes the isolation of 3,3′,4′-tri-O-methyl-quercetin, 3,7,3′,4′-tetra-O-methyl-quercetin (retusin), and 3,7-di-O-methyl-kaempferol (kumatakenin) from the dichloromethane extract of leaves of Siparuna cristata (Poepp. & Endl.) A.DC., Siparunaceae, using high-speed countercurrent chromatography in addition to the investigation of their inhibitory effect against SARS-CoV-2 viral replication. Retusin and kumatakenin inhibited SARS-CoV-2 replication in Vero E6 and Calu-3 cells, with a selective index greater than lopinavir/ritonavir and chloroquine, used as control. Flavonoids and their derivatives may stand for target compounds to be tested in future clinical trials to enrich the drug arsenal against coronavirus infections.
Databáze: OpenAIRE
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