Could induced apoptosis of human granulosa cells predict in vitro fertilization–embryo transfer outcome?
| Autor: | Jean-Louis Benifla, Gérard Feldmann, Patrick Madelenat, Raphaël Porcher, Christophe Sifer, Grazielle Blanc-Layrac, Annie-France Bringuier |
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| Rok vydání: | 2002 |
| Předmět: |
endocrine system
medicine.medical_specialty In vitro fertilisation business.industry Granulosa cell medicine.medical_treatment Obstetrics and Gynecology Embryo transfer Pregnancy rate chemistry.chemical_compound Endocrinology Reproductive Medicine chemistry Apoptosis Internal medicine Follicular phase medicine Interferon gamma sense organs DAPI business hormones hormone substitutes and hormone antagonists medicine.drug |
| Zdroj: | European Journal of Obstetrics & Gynecology and Reproductive Biology. 103:150-153 |
| ISSN: | 0301-2115 |
| DOI: | 10.1016/s0301-2115(02)00043-x |
| Popis: | Objective: The aim of this study was to investigate the relationship between induced apoptosis of human luteinized granulosa cells (GCs) and in vitro fertilization (IVF) outcome. Study design: We induced apoptosis with interferon gamma and an anti-human Fas antibody in cultured GCs isolated from follicular fluids coming from 25 different women undergoing 25 consecutive IVF cycles. After examination of 1000 GCs stained by DAPI with a fluorescent microscope, we determined the percentages of apoptotic GCs. Ovarian, endometrial and IVF parameters were recorded for every woman. Results: We classified the women according to their induced GCs apoptosis percentages in two groups. Group 1 with a low percentage of apoptotic GCs (11.6±4.8%) had a significantly higher pregnancy rate (P |
| Databáze: | OpenAIRE |
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