Indirect determination of phytic acid in urine

Autor:	J. G. March, Felix Grases, Amparo Salvador, B. M. Simonet
Rok vydání:	1998
Předmět:	Phytic acid Chromatography Ion exchange Chemistry Ion chromatography Extraction (chemistry) Aqueous two-phase system food and beverages Biochemistry Analytical Chemistry Hydrolysis chemistry.chemical_compound Molybdenum blue Environmental Chemistry Quantitative analysis (chemistry) Spectroscopy
Zdroj:	Analytica Chimica Acta. 367:63-68
ISSN:	0003-2670
DOI:	10.1016/s0003-2670(98)00164-0
Popis:	An indirect spectrophotometric method for the determination of phytic acid in human urine based on the reduction of molybdophosphate to molybdenum blue and its extraction as an ion pair with Adogen 464 is described. The method requires a clean-up of the sample with activated carbon, a separation of phytic acid from other components by column anion exchange chromatography, and its hydrolysis by microwave treatment. The calibration graph was linear over the range 0.03–0.30 mg l−1 PO3−4 (referred to the aqueous phase), its slope being 1.66 l mg−1. The relative standard deviation for a sample containing 6.4 mg l−1 phytic acid was 0.7% (n=5). Found concentrations in urine were in the range 6.4–0.5 mg l−1 phytic acid.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::94de8919637002fc456608d98f70bbe2 https://doi.org/10.1016/s0003-2670(98)00164-0 Zobrazit plný text záznamu Full Text from ScienceDirect