Modifications in major satellite methylation in the nucleus of a two-cell mouse embryo with respect to developmental conditions

Autor: A. P. Dyban, Inna S. Kuznetsova, E. M. Noniashvili, E. V. Gavrilova
Rok vydání: 2009
Předmět:
Zdroj: Russian Journal of Developmental Biology. 40:305-312
ISSN: 1608-3326
1062-3604
DOI: 10.1134/s1062360409050051
Popis: A study was conducted of the degree of DNA methylation in the nucleus, in particular, of the major satellite in two-cell mouse embryos developing in the maternal organism, in standard culture medium M16, used for cultivating mouse embryos; and M2 media used for manipulating embryos in air. Two-cell embryo nuclei at 44–46 h after injections of chorionic hormone were investigated. The results are evidence for the dependence of the major satellite’s methylation level on the developmental conditions of embryos. The methylation level of the nuclear DNA was shown to increase with a deterioration of environmental conditions. It was reported that in the case of cultivation in M2 media unsuitable for long cultivation, the DNAis methylation level, the major satellite in particular, was higher compared to other embryo groups. Accordingly, not only a significant number of genes but also sequences of satellite DNA are involved in epigenetic regulation.
Databáze: OpenAIRE
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