A quick, easy and cost-effective in planta method to develop direct transformants in wheat

Autor: Amolkumar U. Solanke, Arul Prakash, Avijit Tarafdar, Meenal Bhati, Jasdeep Chatrath Padaria, Harinder Vishwakarma, Sellamuthu Gothandapani, Koushik Biswas, Uttara Chaturvedi
Rok vydání: 2019
Předmět:
Zdroj: 3 Biotech. 9
ISSN: 2190-5738
2190-572X
DOI: 10.1007/s13205-019-1708-6
Popis: Agrobacterium mediated in planta method was used to transform Indian elite wheat genotype HD2894 with herbicide-tolerant CP4-EPSPS (5-enolpyruvylshikimate-3-phosphate synthase) gene. The apical meristems of germinated seeds were targeted for introgression of transgene. The obtained T1 plants were screened by spraying 1% glyphosate and only positive transformants survived. The presence of transgene was also confirmed by PCR and Southern hybridization. Using this method, 3.07% transformation rate was observed. To identify transgenic lines carrying stably integrated CP4-EPSPS gene, the transgenic populations were screened in T3 generation using 1% glyphosate and lines with 100% survival were considered as homozygous. No significant morpho-physiological variations were observed within the transgenic lines as compared to non-transgenic plants. The present study resulted in herbicide-tolerant transgenic wheat and provides a valuable tool for development of wheat genetic transformation.
Databáze: OpenAIRE
Externí odkaz: