514-P: Group 2 Innate Lymphoid Cells Promote Renal Fibrosis through the Production of Th2 Cytokines in Diabetic Kidney Disease

Autor:	Chenlin Gao, Zongzhe Jiang, Ting Zhang, Lu Dan Qin, Jing Ya Ding, Luping Zhou, Man Guo, Yong Xu, Cuiping Liu, Wei Huang
Rok vydání:	2019
Předmět:	biology medicine.diagnostic_test Diabetic kidney business.industry Endocrinology Diabetes and Metabolism Innate lymphoid cell Disease Type 2 diabetes medicine.disease Flow cytometry Fibronectin Pathogenesis Immunology Internal Medicine Renal fibrosis medicine biology.protein business
Zdroj:	Diabetes. 68
ISSN:	1939-327X 0012-1797
DOI:	10.2337/db19-514-p
Popis:	Aim: To explore the role of Group 2 innate lymphoid cells (ILC2s) in the pathogenesis of renal fibrosis in diabetic kidney disease (DKD). Methods: The proportion of ILC2s and ILC2s-producted Th2 cytokines (IL-4, IL-5, IL-13) in the peripheral blood of normal control subjects (NC) or patients with type 2 diabetes (DM), early diabetic kidney disease (DKD1), or late diabetic kidney disease (DKD2) were analyzed using flow cytometry and ELISA. Renal tubular epithelial cells (HK-2) were stimulated with IL-4 or IL-13 in the presence or absence of high glucose and the expression and release of transforming growth factor-β1 (TGF-β1) and fibronectin (FN) was analyzed using qPCR and ELISA. Results: The proportion of ILC2s and the level of IL-4, IL-5, and IL-13 were significantly increased with the severity of DKD (P Conclusions: ILC2s may promote the development and progression of diabetic kidney disease by secreting Th2 cytokines (IL-4 and IL-13) and promoting renal fibrosis. Disclosure C. Liu: None. L. Zhou: None. C. Gao: None. T. Zhang: None. M. Guo: None. W. Huang: None. Z. Jiang: None. J.Y. Ding: None. Y. Xu: None.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::9440d23b116d15eb1e251884b8975253 https://doi.org/10.2337/db19-514-p Zobrazit plný text záznamu