Analysis of Four Chitin-Active Lytic Polysaccharide Monooxygenases from Streptomyces griseus Reveals Functional Variation
| Autor: | Gustav Vaaje-Kolstad, Yuko Nakagawa, Madoka Kudo, Takeshi Watanabe, Kazuhide Totani, Reiya Onodera, Lily Zuin Ping Ang, Vincent G. H. Eijsink |
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| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
chemistry.chemical_classification biology fungi 010401 analytical chemistry macromolecular substances General Chemistry Monooxygenase Chitobiose biology.organism_classification Polysaccharide 01 natural sciences 0104 chemical sciences carbohydrates (lipids) chemistry.chemical_compound Enzyme chemistry Chitin Biochemistry Serratia marcescens Chitinase biology.protein General Agricultural and Biological Sciences Streptomyces griseus 010606 plant biology & botany |
| Zdroj: | Journal of Agricultural and Food Chemistry. 68:13641-13650 |
| ISSN: | 1520-5118 0021-8561 |
| Popis: | Lytic polysaccharide monooxygenases (LPMOs) are redox-active enzymes that cleave insoluble polysaccharides by an oxidative reaction. In the present study, we have characterized four recombinant putative chitin-active LPMOs from Streptomyces griseus (SgLPMO10B, -C, -D, and -F) and evaluated their potential in enhancing hydrolysis of α- and β-chitin by three families of 18 chitinases of Serratia marcescens, SmChiA, -B, and -C. All four recombinant SgLPMO10s showed oxidative activity toward both α- and β-chitin but exhibited different abilities to promote the release of chitobiose from chitin by chitinases depending on both the chitinase and the chitin type. These effects were observed under conditions where the amount of LPMO in the reaction was not rate-limiting, showing that the observed functional differences relate to different abilities of the LPMOs to interact with and act on the substrate. These results show that four seemingly similar LPMOs carrying out the same reaction, cleavage of chitin by C1 oxidation, may have different roles in natural chitin conversion, which provides a rationale for the multiplicity of these enzymes within the same organism. The ability of the LPMOs to act on more natural substrates was demonstrated by showing that SgLPMO10B improved chitin solubilization in dried powdered shrimp shells. |
| Databáze: | OpenAIRE |
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