| Autor: | In Kyu Lee, Jung Eun Ahn, Chul Soo Shin, Chulyoung Kim |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
Chymotrypsin
Dipeptide biology Mixing (process engineering) Substrate (chemistry) Bioengineering General Medicine Mole fraction Applied Microbiology and Biotechnology Kyotorphin chemistry.chemical_compound chemistry Amide biology.protein Organic chemistry Biotechnology Eutectic system Nuclear chemistry |
| Zdroj: | Biotechnology Letters. 23:1423-1427 |
| ISSN: | 0141-5492 |
| DOI: | 10.1023/a:1011678132456 |
| Popis: | The kyotorphin precursor, N-carbobenzoxyl-l-tyrosine-l-arginine amide (N-CBZ-l-Tyr-l-ArgNH2), was synthesized from N-CBZ-l-tyrosine ethyl ester (N-CBZ-l-TyrOEt) and l-arginine amide (l-ArgNH2) by using α-chymotrypsin. Eutectic mixtures were formed by mixing the substrates in the presence of water and/or organic solvents as adjuvants. The eutectic temperature was obtained with a 0.45 l-ArgNH2 mole fraction. It was lowered to 23 °C by addition of 10% (v/w) water and 5% (v/w) dimethyl formamide, thus maintaining homogeneous liquid states at the reaction temperature of 30 °C. After 9 h the solutions became solidified and no further reaction took place. Approximately 90% (mol/mol) conversion was achieved from the substrate mixtures with substrate contents responsible for more than 80% (w/w) of the total mixture. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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