| Autor: |
Maija-Riitta Hirvonen, Jarno Ruusunen, Stefanie Kasurinen, Jorma Jokiniemi, Miko Happo, Hanna Koponen, Oskari Uski, Kari E. J. Lehtinen, Olli Väisänen, Olli Sippula, Die Fang, Pasi Jalava, Cheng Gu, Antti Ruuskanen, Qin'geng Wang, Kari Kuuspalo, Mika Komppula, Liqing Hao, Tiina Torvela |
| Rok vydání: |
2015 |
| Předmět: |
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| Zdroj: |
Atmospheric Environment. 120:427-437 |
| ISSN: |
1352-2310 |
| Popis: |
Urban air particulate pollution is a known cause for adverse human health effects worldwide. China has encountered air quality problems in recent years due to rapid industrialization. Toxicological effects induced by particulate air pollution vary with particle sizes and season. However, it is not known how distinctively different photochemical activity and different emission sources during the day and the night affect the chemical composition of the PM size ranges and subsequently how it is reflected to the toxicological properties of the PM exposures. The particulate matter (PM) samples were collected in four different size ranges (PM10-2.5; PM2.5-1; PM1-0.2 and PM0.2) with a high volume cascade impactor. The PM samples were extracted with methanol, dried and thereafter used in the chemical and toxicological analyses. RAW264.7 macrophages were exposed to the particulate samples in four different doses for 24 h. Cytotoxicity, inflammatory parameters, cell cycle and genotoxicity were measured after exposure of the cells to particulate samples. Particles were characterized for their chemical composition, including ions, element and PAH compounds, and transmission electron microscopy (TEM) was used to take images of the PM samples. Chemical composition and the induced toxicological responses of the size segregated PM samples showed considerable size dependent differences as well as day to night variation. The PM10-2.5 and the PM0.2 samples had the highest inflammatory potency among the size ranges. Instead, almost all the PM samples were equally cytotoxic and only minor differences were seen in genotoxicity and cell cycle effects. Overall, the PM0.2 samples had the highest toxic potential among the different size ranges in many parameters. PAH compounds in the samples and were generally more abundant during the night than the day, indicating possible photo-oxidation of the PAH compounds due to solar radiation. This was reflected to different toxicity in the PM samples. Some of the day to night difference may have been caused also by differing wind directions transporting air masses from different emission sources during the day and the night. The present findings indicate the important role of the local particle sources and atmospheric processes on the health related toxicological properties of the PM. The varying toxicological responses evoked by the PM samples showed the importance of examining various particle sizes. Especially the detected considerable toxicological activity by PM0.2 size range suggests they're attributable to combustion sources, new particle formation and atmospheric processes. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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