Expression of SOD and production of reactive oxygen species in Acinetobacter calcoaceticus caused by hydrocarbon oxidation
| Autor: | M. A. Sazykina, Maria Zhuravlevа, E. M. Kudeevskaya, Shorena Karchava, L. E. Khmelevtsova, I. S. Sazykin, M. I. Khammami |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
chemistry.chemical_classification Reactive oxygen species Antioxidant biology Superoxide medicine.medical_treatment 030106 microbiology biology.organism_classification Applied Microbiology and Biotechnology Superoxide dismutase 03 medical and health sciences chemistry.chemical_compound 030104 developmental biology Hydrocarbon Biotransformation chemistry Biochemistry Catalase biology.protein medicine Acinetobacter calcoaceticus |
| Zdroj: | Annals of Microbiology. 66:1039-1045 |
| ISSN: | 1869-2044 1590-4261 |
| Popis: | Expression of antioxidant enzymes [superoxide dismutase (SOD) and catalase] and generation of reactive oxygen species (ROS) in the presence of various hydrocarbons were investigated in two strains of hydrocarbon-degrading microorganism Acinetobacter calcoaceticus isolated from water (VKPM B-10353) and bottom sediment (EMBM-06). Upon biotransformation of hydrocarbons by the studied strains, superoxide generation strengthening by bacterial cells and accumulation of peroxides in the cultivation medium were observed. The strongest superoxide anion radical generation occurred during the incubation of microorganisms with diesel fuel (increasing 12 times and 2 times), cyclohexane (1.8 times and 1.75 times), a mix of benzene and anthracene (4.4 times and 3.7 times), naphthalene (8 times and 1.9 times) and benz(a)pyrene (2 times and 2.3 times) in strains VKPM B-10353 and EMBM-06 respectively, and anthracene (2.8 times) in strain VKPM B-10353. The accumulation of peroxides in the medium took place upon biotransformation of diesel fuel by both strains. Activity of SOD increased considerably and activity of catalase decreased in cells in the medium with hydrocarbons. Decane and crude oil had the maximum impact on SOD expression. Decane increased SOD expression in strains VKPM B-10353 and EMBM-06 24.4 times and 28.5 times, respectively. Crude oil increased SOD expression in strain VKPM B-10353 19 times, and in strain EMBM-06 16 times. The authors assumed that increased ROS generation and oxidizing stress development in bacteria during the biotransformation of various hydrocarbons can have an adaptive character. |
| Databáze: | OpenAIRE |
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