Application of novel magnetizable cellulose particles in the development of immunoradiometric assay for C-peptide
| Autor: | Jayula Sarnaik, R. R. Rasmi, H.M. Somashekarappa, V. B. Kadwad, K. Bhasker Shenoy |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
Immunoradiometric assay
Chromatography Chemistry medicine.drug_class Health Toxicology and Mutagenesis Wet grinding Public Health Environmental and Occupational Health Monoclonal antibody Pollution Analytical Chemistry chemistry.chemical_compound Cross matching Nuclear Energy and Engineering medicine Radiology Nuclear Medicine and imaging Cellulose Spectroscopy |
| Zdroj: | Journal of Radioanalytical and Nuclear Chemistry. 304:1115-1122 |
| ISSN: | 1588-2780 0236-5731 |
| DOI: | 10.1007/s10967-015-3925-8 |
| Popis: | The present study was oriented to develop, optimize and validate immunoradiometric assay for the measurement of C-peptide in human serum by employing magnetizable cellulose particles prepared by a novel method of wet grinding. Six commercial monoclonal antibodies raised against the different epitopes of C-peptide were evaluated for their compatibility as sandwich partners by “cross matching”. The developed one step inclusive assay with best matched combination of sandwich complex, “magnetizable cellulose particles linked capture antibody–C-peptide–125I labelled detector antibody” had an analytical sensitivity of 0.16 ng/ml and standard range of 0.3–30 ng/ml covering required physiological and diagnostic range. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|