Development of real‐time PCR assays for the detection of Moraxella macacae associated with bloody nose syndrome in rhesus ( Macaca mulatta ) and cynomolgus ( Macaca fascicularis ) macaques

Autor:	Timothy D. Minogue, Monica E. Embers, Gustavo Palacios, Kitty Chase, Chris A. Whitehouse, David A. Kulesh, Jason T. Ladner
Rok vydání:	2015
Předmět:	General Veterinary Biology Molecular diagnostics biology.organism_classification Virology Moraxella macacae Bloody medicine.anatomical_structure Real-time polymerase chain reaction Nasal Swab medicine Animal Science and Zoology Pathogen Moraxella Nose
Zdroj:	Journal of Medical Primatology. 44:364-372
ISSN:	1600-0684 0047-2565
Popis:	Background Moraxella macacae is a recently described bacterial pathogen that causes epistaxis or so-called bloody nose syndrome in captive macaques. The aim of this study was to develop specific molecular diagnostic assays for M. macacae and to determine their performance characteristics. Methods We developed six real-time PCR assays on the Roche LightCycler. The accuracy, precision, selectivity, and limit of detection (LOD) were determined for each assay, in addition to further validation by testing nasal swabs from macaques presenting with epistaxis at the Tulane National Primate Research Center. Results All assays exhibited 100% specificity and were highly sensitive with an LOD of 10 fg for chromosomal assays and 1 fg for the plasmid assay. Testing of nasal swabs from 10 symptomatic macaques confirmed the presence of M. macacae in these animals. Conclusions We developed several accurate, sensitive, and species-specific real-time PCR assays for the detection of M. macacae in captive macaques.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::90d6728efb4c6fc0235330add4e2e38c https://doi.org/10.1111/jmp.12196 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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