Effects of therapeutic ultrasound on fetal limb development in an organ culture system

Autor:	Pamela E. Houghton, Anna Radman
Rok vydání:	2000
Předmět:	Fetus medicine.medical_specialty Therapeutic ultrasound business.industry medicine.medical_treatment Pulsed Ultrasound Ultrasound Physical Therapy Sports Therapy and Rehabilitation Fetal limb Organ culture Surgery Fetal mouse medicine Nuclear medicine business
Zdroj:	Physiotherapy Theory and Practice. 16:119-134
ISSN:	1532-5040 0959-3985
DOI:	10.1080/095939800750036051
Popis:	The use of therapeutic ultrasound by physical therapists on pregnant women is currently contraindicated due to unknown effects it may have on the developing fetus. This study examines the effects of therapeutic ultrasound (US) on the development of fetal mouse limbs in an in vitro organ culture system by placing mouse limbs (N 241) in a culture and exposing them to daily US treatments lasting 5 minutes for 4 days at a frequency of 3 MHz and one of the following intensities (SATP) : 1.0 W/cm2 and 100% duty cycle (CONT), 1.0 W/cm2 (1.0 PULSED) or 0.1 W/cm2 (0.1 PULSED) at 20% duty cycle, and sham controls (SHAM). Limbs were examined histologically and serial cross sections were rated by blinded observers using a predetermined criteria for relative limb development. The percentage of nonviable limbs was elevated in limbs treated with 1.0 PULSED ultrasound and was two times higher after treatments with CONT ultrasound. Quantitative analysis of collagen distribution was performed using computerized image analy...
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::8ee88828d69812513d1b4da6d973e0ae https://doi.org/10.1080/095939800750036051 Zobrazit plný text záznamu Plný text ve formátu PDF