Exposure of rod outer segments to serum is not responsible for abnormal disk membrane morphogenesis in a model of retinal detachment
| Autor: | Michael W. Kaplan |
|---|---|
| Rok vydání: | 1998 |
| Předmět: |
Retina
Retinal pigment epithelium genetic structures biology Albumin Morphogenesis Xenopus Retinal detachment Retinal Anatomy biology.organism_classification medicine.disease eye diseases Sensory Systems Epithelium Cell biology Cellular and Molecular Neuroscience Ophthalmology chemistry.chemical_compound medicine.anatomical_structure chemistry medicine sense organs |
| Zdroj: | Current Eye Research. 17:793-797 |
| ISSN: | 1460-2202 0271-3683 |
| DOI: | 10.1080/02713689808951260 |
| Popis: | Purpose. The sclerad surface of the retina is exposed to serum proteins in several retinal pathologies that result in degeneration of photoreceptor outer segments. Abnormal disk membrane morphogenesis is observed in rod photoreceptors of detached Xenopus retinas when they are cultured in serum-containing medium. Retinas that remain attached to the pigment epithelium layer produce normal disks. Experiments were conducted to determine whether abnormal disk morphogenesis in detached, cultured retinas is due to the presence of serum in the microenvironment of the rod outer segments.Methods. Detached retinas and retinas attached to the retinal pigment epithelium in eyecups were cultured in either serum-containing or serum-free medium, and the morphology of the disk lamellae formed in vitro retinas was evaluated. Using protein extraction and immunochemical methods, the presence of albumin in the microenvironment of the outer segments was confirmed for retinas incubated in serum-containing medium.Results. There ... |
| Databáze: | OpenAIRE |
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