The Biosynthesis of the Pyrimidine Moiety of Thiamin in Halobacterium salinarum
| Autor: | Keiko Tazuya-Murayama, Yukie Kijima, Kazuko Yamada, Maria Hayashi |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Nutrition and Dietetics Ribonucleotide Pyrimidine biology Medicine (miscellaneous) 010402 general chemistry biology.organism_classification medicine.disease_cause 01 natural sciences 0104 chemical sciences 03 medical and health sciences chemistry.chemical_compound 030104 developmental biology chemistry Biochemistry Biosynthesis Halobacterium salinarum medicine Thiazole Purine metabolism Escherichia coli Histidine |
| Zdroj: | Journal of Nutritional Science and Vitaminology. 62:130-133 |
| ISSN: | 1881-7742 0301-4800 |
| DOI: | 10.3177/jnsv.62.130 |
| Popis: | The biosynthetic pathway of the pyrimidine moiety of thiamin was studied in the archaean Halobacterium salinarum. Thiamin is biosynthesized from 4-amino-5-hydroxymethyl-2-methylpyrimidine (pyrimidine) and 5-(2-hydroxyethyl)-4-methylthiazole (thiazole). The pyrimidine and the thiazole are biosynthesized de novo in microorganisms. The biosynthetic routes of pyrimidine in microorganisms differ between eukaryote and eubacteria. In the eukaryote Saccharomyces cerevisiae, histidine and pyridoxine are the precursors of pyrimidine, while in the eubacterium Escherichia coli, pyrimidine is biosynthesized from 5-aminoimidazole ribonucleotide (AIR), an intermediate of purine biosynthesis. Tracer investigations revealed that [(15)N]-, [1-(13)C]- and [2-(13)C] glycine, precursors of AIR, were incorporated into the pyrimidine in H. salinarum. These results suggested that the biosynthetic route of the pyrimidine in H. salinarum is similar to that of E. coli. |
| Databáze: | OpenAIRE |
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