Characterization of chromatin from Triticum aestivum

Autor:	R.J. Kemble, R.B. Flavell
Rok vydání:	1974
Předmět:	biology Sodium Ion chromatography chemistry.chemical_element RNA Plant Science General Medicine Horticulture Biochemistry Molecular biology Chromatin chemistry.chemical_compound Histone chemistry biology.protein Urea Molecular Biology Polyacrylamide gel electrophoresis DNA
Zdroj:	Phytochemistry. 13:1689-1694
ISSN:	0031-9422
DOI:	10.1016/0031-9422(74)85074-0
Popis:	Chromatin was isolated from wheat seedlings using chromatin aggregation in the presence of cations and dispersion in the absence of these cations. The preparation contained some contaminating RNA. The protein, DNA and RNA moieties were separated from each other by ion exchange chromatography on hydroxyapatite in the presence of 5 M urea and 2 M NaCl. The histone and non-histone proteins were subsequently separated on CM-cellulose and the histones partially separated from one another. The various protein fractions were characterized by urea-acetic acid and sodium dodecyl sulphate polyacrylamide gel electrophoresis.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::8c554ee6f94b93e8b54687d4940757ba https://doi.org/10.1016/0031-9422(74)85074-0 Zobrazit plný text záznamu