An MspI PCR-RFLP detecting a single nucleotide polymorphism at alpha-lactalbumin gene in goat
| Autor: | Zhang, C.L.,Northwest A, C.Z. Lei, Pan, C.Y.,Northwest A, Li, J.Y.,Northwest A, Chen, H.,Northwest A, Lan, X.Y.,Northwest A, Zhang, L.,Northwest A, Zhang, A.L.,Northwest A |
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| Rok vydání: | 2007 |
| Předmět: |
0301 basic medicine
Genetics biology 0402 animal and dairy science Single-nucleotide polymorphism Locus (genetics) 04 agricultural and veterinary sciences 040201 dairy & animal science 03 medical and health sciences LALBA Gene 030104 developmental biology Genotype Alpha-lactalbumin biology.protein Animal Science and Zoology Restriction fragment length polymorphism Allele Identity by type |
| Zdroj: | Czech Journal of Animal Science. 52:138-142 |
| ISSN: | 1805-9309 1212-1819 |
| DOI: | 10.17221/2231-cjas |
| Popis: | Alpha-lactalbumin (α-LA, LALBA) was strongly correlated with the nutritional value and the functional properties of whey and whey products. However, there are not many studies of goat LALBA gene and its polymorphisms in literary sources. In this paper, on the basis of PCR-SSCP and DNA sequencing, one M63868:g.1897T>C mutation in exon 3 at LALBA locus identified a Single Nucleotide Polymorphism (SNP): p.L100P of the LALBA protein, which could be detected by MspI endonuclease. Hence, we described a method based on MspI PCR-RFLP for SNP detection at the LALBA locus in 801 goats of China. Frequencies of goat LALBA-A2 allele varied from 0.000 to 0.024 in Chinese populations. Gene heterozygosity, effective allele numbers and PIC (Polymorphism Information Content) of goat LALBA locus in Chinese populations varied from 0.000 to 0.0472, 1.000 to 1.0496, 0.000 to 0.0461, respectively, which were lower values than in Italian populations. Therefore, we considered that the low frequency of LALBA-A2 allele at goat LALBA gene possibly characterized Chinese native breeds. Moreover, Chinese native populations demonstrated poorer polymorphism at goat LALBA locus than Italian populations. |
| Databáze: | OpenAIRE |
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