Isolation and characterisation of 20 microsatellite loci from the Chinese green tree viper Trimeresurus stejnegeri stejnegeri
| Autor: | Hua Ching Lin, Shou Hsien Li, Ming Hsung Chang, Yi Chun Chang |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0106 biological sciences
0301 basic medicine Genetics biology Trimeresurus biology.organism_classification 010603 evolutionary biology 01 natural sciences Southeast asia Loss of heterozygosity 03 medical and health sciences 030104 developmental biology Trimeresurus stejnegeri Chinese green tree viper Microsatellite Polymorphic Microsatellite Marker Animal Science and Zoology Allele Ecology Evolution Behavior and Systematics |
| Zdroj: | Amphibia-Reptilia. 37:126-130 |
| ISSN: | 1568-5381 |
| Popis: | The Chinese green tree viper (Trimeresurus stejnegeri stejnegeri), one of the most common snakes in Southeast Asia, can be a good model species for evolutionary and behavioral research. However, there is no high polymorphic co-dominant marker that can be used for individual-based genetic analyses available for this species. Therefore, we developed 20 polymorphic microsatellite loci for T. s. stejnegeri in Taiwan by screening a microsatellite-enriched DNA library. The allele numbers of these loci ranged from 3 to 22, and the observed heterozygosity were 0.042-1.000. The probability of false parent non-exclusion was 2 × 10−5 for those loci that conformed to the Hardy–Weinberg equilibrium. These highly polymorphic microsatellite markers are useful for both individual-based and population-level analysis. Furthermore, this is the first set of microsatellite markers developed for Trimeresurus, and could be applied to closely related species to address various research questions on evolution and animal behavior. |
| Databáze: | OpenAIRE |
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