The effect of pH on the high pH anion-exchange chromatography elution of monosaccharides

Autor:	J. M. Mcguire, Yvonne M. Stewart, Kevin D. Smith
Rok vydání:	1999
Předmět:	chemistry.chemical_classification Chromatography Ion exchange Elution Organic Chemistry Clinical Biochemistry Ion chromatography Mannose Biochemistry Analytical Chemistry chemistry.chemical_compound chemistry Sodium hydroxide Galactosamine Galactose Monosaccharide
Zdroj:	Chromatographia. 49:699-702
ISSN:	1612-1112 0009-5893
DOI:	10.1007/bf02466915
Popis:	We have studied the elution behaviour of six common monosaccharides, fucose, galactosamine, glucosamine, galactose, glucose and mannose, on the CarboPac PA-100 column. The relative elution positions of galactose, glucose and mannose were dependent on the sodium hydroxide concentrations, a phenomenon which was likely to be the result of differential ionisation of the hydroxyl groups at ring positions two and four. The optimal resolution of the monosaccharides studied was achieved by elution with 30 mM sodium hydroxide with no appreciable loss in sensitivity at this low concentration.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::8be7162b9bb3916e660f4ada80628c02 https://doi.org/10.1007/bf02466915 Zobrazit plný text záznamu Full text from SpringerLink