Electrophoretic investigation of the histones of the cotton plant in polyacrylamide gel containing sodium dodecylsulfate

Autor: N. I. Koryakina, G. F. Kasymova, V. K. Burichenko, T. P. Konitsya
Rok vydání: 1981
Předmět:
Zdroj: Chemistry of Natural Compounds. 17:349-351
ISSN: 1573-8388
0009-3130
DOI: 10.1007/bf01185264
Popis: In order to study the fractional compositions of the histones isolated from the cotton plant, they have been subjected to electrophoretic separation in polyacrylamide gels (PAAGs) containing sodium dodecylsulfate (NA-DDS). It has been established that the rate and sequence of migration of the histones under consideration in PAAGs differ from the corresponding values of the histones of the calf thymus. A comparative evaluation of the histones of the cotton plant and of the calf thymus has been made by two-dimensional electrophoresis. The molecular weights of fractions of the cotton-plant histone were determined from their electrophoretic mobilities in PAAGs with Na-DDS:H1 - 23,500;H2A - 14,600; H2B - 14,960; H3 - 14,300; and H4 - 11,300.
Databáze: OpenAIRE