Electrophoretic investigation of the histones of the cotton plant in polyacrylamide gel containing sodium dodecylsulfate
Autor: | N. I. Koryakina, G. F. Kasymova, V. K. Burichenko, T. P. Konitsya |
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Rok vydání: | 1981 |
Předmět: |
Chromatography
biology Molecular mass Polyacrylamide Plant Science General Chemistry General Biochemistry Genetics and Molecular Biology Comparative evaluation Electrophoresis chemistry.chemical_compound Histone chemistry Biochemistry biology.protein Electrophoretic mobilities Polyacrylamide gel electrophoresis Sodium dodecylsulfate |
Zdroj: | Chemistry of Natural Compounds. 17:349-351 |
ISSN: | 1573-8388 0009-3130 |
DOI: | 10.1007/bf01185264 |
Popis: | In order to study the fractional compositions of the histones isolated from the cotton plant, they have been subjected to electrophoretic separation in polyacrylamide gels (PAAGs) containing sodium dodecylsulfate (NA-DDS). It has been established that the rate and sequence of migration of the histones under consideration in PAAGs differ from the corresponding values of the histones of the calf thymus. A comparative evaluation of the histones of the cotton plant and of the calf thymus has been made by two-dimensional electrophoresis. The molecular weights of fractions of the cotton-plant histone were determined from their electrophoretic mobilities in PAAGs with Na-DDS:H1 - 23,500;H2A - 14,600; H2B - 14,960; H3 - 14,300; and H4 - 11,300. |
Databáze: | OpenAIRE |
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