A simple, efficient agroinoculation soaking procedure for Tomato yellow leaf curl virus

Autor:	Koji Miyatake, Hiroyuki Fukuoka, Jun Ohnishi, Hirotaka Yamaguchi, Akio Ohyama, Satomi Negoro, Tsukasa Nunome
Rok vydání:	2013
Předmět:	Soaking Procedure biology Agrobacterium fungi Begomovirus food and beverages Plant Science biology.organism_classification Virus Family Geminiviridae Horticulture Shoot Botany Cultivar Tomato yellow leaf curl virus Agronomy and Crop Science
Zdroj:	Journal of General Plant Pathology. 79:243-248
ISSN:	1610-739X 1345-2630
DOI:	10.1007/s10327-013-0450-x
Popis:	Tomato yellow leaf curl virus (TYLCV, genus Begomovirus, family Geminiviridae), poses a serious threat to tomato crops in tropical and subtropical regions. We developed a simple agroinoculation method with an infectious clone of TYLCV. Dipping of excised sections of susceptible tomato shoots in an Agrobacterium suspension successfully introduced the replicating virus with high efficiency. An additional vacuum treatment for 5 min ensured uniform infection without escapes, allowing evaluation of differences in TYLCV resistance among tomato cultivars. The method can be used in laboratory experiments for virological studies and in breeding programs for resistant cultivars.
Databáze:	OpenAIRE
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