AnIsaria fumosoroseaSCAR marker for evaluation of soil, insect, and airborne samples

Autor:	Miguel Ángel Ayala-Zermeño, T. Robledo-Retana, Conchita Toriello, E. Arroyo-Vázquez, Teresa Mier, María del Carmen Calderón-Ezquerro, María del Rocío Reyes-Montes
Rok vydání:	2011
Předmět:	Entomopathogenic fungi biology media_common.quotation_subject Fungus Insect biology.organism_classification Microbiology Paecilomyces carneus Insect Science Metarhizium acridum Lecanicillium lecanii Agronomy and Crop Science Isaria fumosorosea media_common
Zdroj:	Biocontrol Science and Technology. 21:1091-1102
ISSN:	1360-0478 0958-3157
Popis:	Molecular detection markers are needed for ecological studies of entomopathogenic fungi. In this study, a sequence-characterized amplified region (SCAR) marker of Isaria fumosorosea was used to detect the fungus in soil, insects, and airborne samples. These were artificially added with different fungal conidial concentrations. Specificity and sensitivity were tested with semi-nested PCR using oligonucleotides E-AA/M-CTA124 F and E-AA/M-CTA124 R for the first amplification and E-AA/M-CTA124 F and E-AA/M-CTA103 R for the second amplification. Specificity assays showed a specific band of 103 bp for DNA samples from 10 I. fumosorosea strains used. Negative results were observed for DNA samples from other species of Isaria, including I. amoene-rosea, I. farinosa, and Paecilomyces carneus as well as with other entomopathogens such as Metarhizium acridum, M. anisopliae, M. majus, M. flavoviride Type E, and Lecanicillium lecanii. Sensitivity assays showed that the specific SCAR marker detected 104 conidi...
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::8b16faa5dd0fbe63cd55bd00cad8197d https://doi.org/10.1080/09583157.2011.603822 Zobrazit plný text záznamu