Cultivation and extraction of omega 3-6 fatty acids from the heterotrophic marine microalga Schizochytrium mangrovei TB17 to make a functional food
| Autor: | Thi Thom Le, Diem Hong Dang |
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| Rok vydání: | 2021 |
| Předmět: | |
| Zdroj: | Research Journal of Biotechnology. 16:22-32 |
| ISSN: | 2278-4535 0973-6263 |
| DOI: | 10.25303/168rjbt2221 |
| Popis: | The objective of this study was to investigate the different fermentation conditions for Schizochytrium mangrovei TB17 biomass production rich in omega-3 and omega-6 fatty acids to determine the optimal conditions for bio-oil extraction from the algal biomass and to study the pharmacological effects of this bio-oil in experimental animal models. After 108 h of fed-batch fermentation, the cell density, dry biomass, lipid contents and docosahexaenoic acid (DHA) contents reached 392.53±3.89×106 cells/mL, 100.41±3.17 g/L, 41.04±0.87% dry cell weight (DCW) and 33.33±1.96% total fatty acids (TFAs) respectively. The in situ transesterification producing bio-oil rich in omega 3-6 fatty acids from the biomass of the TB17 strain reached a yield of TFAs of 45.02±1.37% DCW. The yields of PUFAs and SFAs were 30.02±0.38% and 69.98±0.53% of TFA respectively. The bio-oil mainly contains DHA and EPA accounting for 74.04±0.25% and 10.97±0.43% of the TFAs respectively. The bio-oil met the Vietnam food safety standard. According to the results of the acute in mice and the subchronic oral toxicity in rats for 90 days, the bio-oil rich in omega 3-6 fatty acids is safe and has the ability to improve memory and learning ability in mice which can be made functional food oil capsules for human. |
| Databáze: | OpenAIRE |
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