Down Modulation of N-myc, Heat-Shock Protein 70, and Nucleolin during the Differentiation of Human Neuroblastoma Cells1
| Autor: | Ken Higashi, Ryoichi Ohya, Tadamasa Murakami, Tohru Tsuda, Hisamitsu Ohmori, Shinobu Akiya, Sadao Gotoh |
|---|---|
| Rok vydání: | 1991 |
| Předmět: | |
| Zdroj: | The Journal of Biochemistry. 110:146-150 |
| ISSN: | 1756-2651 0021-924X |
| DOI: | 10.1093/oxfordjournals.jbchem.a123533 |
| Popis: | Cultured human neuroblastoma (GOTO) cells were induced to differentiate by dibutyryl cyclic AMP (Bt2cAMP) and/or retinoic acid (RA). A combination of Bt2cAMP (1 mM) and RA (1 microM) yielded the most significant networks of neurites after 3 to 4 days, this being associated with the reduction of N-myc mRNA levels. Next, we examined several cellular genes that were possibly linked with changes in N-myc gene expression under these conditions. Among the genes examined, both nucleolin and a major heat-shock protein (hsp70) mRNAs showed changes concomitant with those in N-myc mRNA levels when induced by Bt2cAMP and RA. Dibutyryl cAMP alone induced several short cellular processes and caused a marked decrease in N-myc mRNA within 2 days. RA alone induced a few long and straight neurites along the longitudinal axis of individual cells and a significant decrease in growth rate but showed neither network formation nor a decrease in N-myc gene expression. These results indicate differential effects of Bt2cAMP and RA on the regulatory mechanisms of both cell proliferation and differentiation and also indicate a possible association of expression of N-myc gene with those of hsp70 and nucleolin genes. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|