Ultracytochemical demonstration of the polarity of Ca2+-ATPase activity in microglia

Autor:	Shigetoshi Kuroda, Kosuke Fukai, Hideki Ishizu, Kensuke Kawai, Yasuyuki Tanabe, Yasunobu Komoto
Rok vydání:	1998
Předmět:	Polarity (international relations) Microglia Chemistry General Medicine Pathology and Forensic Medicine Cell biology Reaction product medicine.anatomical_structure nervous system Parenchyma Immunology medicine Atpase activity Lead salt Basal lamina Neurology (clinical) Perivascular Cell
Zdroj:	Neuropathology. 18:284-288
ISSN:	1440-1789 0919-6544
DOI:	10.1111/j.1440-1789.1998.tb00116.x
Popis:	In order to investigate plasma membrane Ca2+-ATPase (Ca2+-ATPase) activity in three subsets of microglia/macrophages (microglia in perineuronal regions (perineuronal microglia)), microglia in contact with the parenchymal side of the vascular basal lamina (juxtavascular microglia) and perivascular cells within the basal lamina), we performed an ultracytochemical study using a lead salt technique in rat cerebral cortices. In 60.6% of perineuronal microglia, the cytochemical reaction (CR) for Ca2+-ATPase on the plasma membrane (PM) in the vicinity of neurons was stronger than the CR on the other side of the PM, and deposits of the reaction product (RP) for Ca2+-ATPase had accumulated, reflecting the polarity of Ca2+-ATPase activity. In contrast, in all of juxtavascular microglia and perivascular cells, deposits of the RP were distributed evenly along the PM without polarity. These results suggest that the polarity of the Ca2+-ATPase activity manifests certain functional interactions between perineuronal microglia and neurons.
Databáze:	OpenAIRE
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