Antigenicity of DNA of bacteriophages

Autor: Z. I. Borishpolets, T. I. Tikhonenko, T. I. Biryulina
Rok vydání: 1966
Předmět:
Zdroj: Bulletin of Experimental Biology and Medicine. 62:1157-1160
ISSN: 1573-8221
0007-4888
DOI: 10.1007/bf00795465
Popis: A study was carried out on the antigenicity of DNA of phages T2 and Sd ofE. coli with the aid of a precipitation reaction by diffusion in gel, complement fixation and sensitization tests. Positive results were obtained only with inadequately purified DNA preparations deproteinized with phenol. Additional deproteinization after Sevag deprived DNA of its antigenic properties. Sera of rabbits immunized with phage DNA gave a positive precipitation reaction with phase protein treated with desoxyribonuclease. Denatured DNA did not show any “latent” antigenic groups. Antibodies formed in the immune sera of animals are most likely the result of protein presence in phage DNA preparations.
Databáze: OpenAIRE
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