Efficient immobilization of whole cells of Methylomonas sp. strain GYJ3 by sol–gel entrapment

Autor:	Jia-Ying Xin, Chun-Gu Xia, Jianbo Chen, Jun-Ru Cui, Shu-Ben Li, Yi Xu
Rok vydání:	2004
Předmět:	Chromatography biology Strain (chemistry) Chemistry Methyltrimethoxysilane Process Chemistry and Technology Bioengineering Sodium silicate biology.organism_classification Biochemistry Methylococcaceae Catalysis chemistry.chemical_compound Entrapment Methylomonas Bacteria Sol-gel
Zdroj:	Journal of Molecular Catalysis B: Enzymatic. 30:167-172
ISSN:	1381-1177
DOI:	10.1016/j.molcatb.2004.05.005
Popis:	The whole cells of Methanotrophic bacteria Methylomonas sp. strain GYJ3 immobilized by the sol–gel technique was investigated in the present work. After the cells were immobilized by entrapment in sodium silicate sol–gel matrix, a higher activity for propylene epoxidation were observed at a common loading amount. In a batch reaction system, the immobilized cells can be repeatedly used for more than 25 times and no significant loss of the activity was found. The activity was well preserved when the immobilized cells were stored at 4 °C for 45 days. Determination of optimal reaction conditions showed that the immobilized cells took on increased activity at a higher pH and temperature. However, the cells entrapped in methyltrimethoxysilane (MTMS) gel matrix gave a lower activity as compared to that of the free cells. Our experimental results indicate that the sol–gel entrapment based on silica matrixes was a simple, efficient and cost-efficient method for the immobilization the whole cells of Methylomonas sp. strain GYJ3 cells.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::8864f471c11eb69922ab81d5802282ed https://doi.org/10.1016/j.molcatb.2004.05.005 Zobrazit plný text záznamu Full Text from ScienceDirect