Online immobilized metal affinity chromatography/mass spectrometric analysis of changes elicited by cCMP in the murine brain phosphoproteome

Autor:	Robin Tuytten, Russell P. Newton, E. L. Esmans, Edward G. Dudley, Shujing Ding, Filip Lemière, A.E. Bond, A.G. Brenton
Rok vydání:	2008
Předmět:	Chromatography CCMP Organic Chemistry Pipette Cytidine Mass spectrometric Analytical Chemistry chemistry.chemical_compound Biochemistry chemistry Murine brain Metal affinity chromatography Phosphorylation Protein phosphorylation Spectroscopy
Zdroj:	Rapid Communications in Mass Spectrometry. 22:4129-4138
ISSN:	0951-4198
DOI:	10.1002/rcm.3834
Popis:	An automated online immobilized metal affinity chromatography/high-performance liquid chromatography mass spectrometric (IMAC-HPLC/MS/MS) method was developed to study cytidine 3',5'-cyclic monophosphate (cCMP)-specific protein phosphorylation, analogous to a previously successful offline IMAC method using microvolume IMAC pipette tips. The optimized method identified murine brain phosphoproteins selectively modified by challenge with cCMP, using manual interpretation of the results to confirm both phosphorylation and selectivity of response to cCMP. A number of proteins identified by this strategy have potential roles in hyperproliferation, a previously reported response to elevated levels of cCMP.
Databáze:	OpenAIRE
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