DIAGNOSTIC BACTERIOPHAGE V32 AS A TOOL FOR THE RAPID IDENTIFICATION OF ESCHERICHIA COLI SEROGROUP O157
| Autor: | A.V. Aleshkin, Edward A. Svetoch, N. V. Volozhantsev, N N Kartsev, Vasily A. Bannov, I.A. Dyatlov, Vera P. Myakinina, I.A. Kiseleva, V. V. Verevkin, V. M. Krasilnikova, Borzenkov Vn |
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| Rok vydání: | 2019 |
| Předmět: |
Serotype
0303 health sciences biology 030306 microbiology Biochemistry (medical) Virulence Myoviridae General Medicine biology.organism_classification medicine.disease_cause Genome Microbiology Bacteriophage 03 medical and health sciences Medical Laboratory Technology fluids and secretions Lytic cycle medicine Bacterial virus Escherichia coli 030304 developmental biology |
| Zdroj: | Russian Clinical Laboratory Diagnostics. 64:57-64 |
| ISSN: | 0869-2084 |
| Popis: | Bacteriophage V32, a representative of bacterial viruses of the Myoviridae family Ounavirinae subfamily, is proposed for search and identification of E. coli O157 serogroup, including Shiga-toxin producing E. coli O157:H7 (STEC O157:H7), among cultures of enterobacteria from the primary seeding of the material studied. Phage genome containes a linear double-stranded DNA of 87875 base pairs with G/C-content of 38.9% and includes 132 open reading frames (ORF). In the genome, there are no determinants of antibiotic resistance, virulence genes of STEC and other well-known pathogroups of E. coli. It has been established that phage V32 has lytic activity against all studied cultures of E. coli O157 serogroup (n=183) isolated from people and farm animals in various regions of the Russian Federation, as well as in Japan and Italy. At the same time, the phage lyses only 6 of 182 strains (3.3%) of E. coli not belonging to the O157 serogroup and is not active against strains of other enterobacteria. That is, the phage has a high specificity. The use of bacteriophage V32 as a diagnostic tool is a highly efficient, fast, cheap and simple method for identifying E. coli serogroup O157, including the serotype E. coli O157: H7, in any bacteriological laboratory without special equipment and special training of performers. |
| Databáze: | OpenAIRE |
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