Cell stress molecules expression in periodontal disease (MUC7P.762)
| Autor: | Luis Aguilera Galaviz, Maria Aceves Medina, Martha Hernandez Montoya, Cristal Diaz Rosas, Silverio Frausto Esparza, Iris Estrada Garcia |
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| Rok vydání: | 2014 |
| Předmět: | |
| Zdroj: | The Journal of Immunology. 192:197.14-197.14 |
| ISSN: | 1550-6606 0022-1767 |
| DOI: | 10.4049/jimmunol.192.supp.197.14 |
| Popis: | Periodontal Disease is bacterial infection of tooth-supporting tissues leading to chronic inflammation and loss of teeth, the immunopathologic mechanisms include inflammatory cells and proinflammatory cytokines; persistent local inflammation develops the expression of stress molecules as Hsp90, Hsp70 and P53 also autoantibodies. The rol of cellular stress and autoimmunity in periodontal disease is not understood. Objetives: Determine the expression of cell stress molecules and auto-antibodies in periodontal disease patients. Methods: 34 patient with severe chronic periodontitis and 14 healthy people were clinical evaluated. Periodontal tissue and sera were obtained. Electroforesis, Western blot and Immunodetection to identify Hsp70, Hsp90 and P53 was set up, Hsp 90 were evaluated by RT-PCR and IFI for ANCA, antiepithelial antibodies in mouse skin and ANA using Hep2 cells. Results: 57% were positives for ANCA, total of sera were positives for nuclear and/or cytoplasmic antibodies and 57% presented anti-epithelial antibodies, Hsp90, Hsp70 and P53 is expressed according with the severity of disease. Conclusions: The expression profile of Hsps in periodontal tissue is according with the severity, and could be associated with cellular stress because of the damage mechanism induced by periodontal pathogens and trigger necrotic or apoptotic phenomena. Destruction of extracellular matrix in periodontal tissue could be mediated by auto-antibodies that recognize self structures. |
| Databáze: | OpenAIRE |
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