Reconstruction of Starch Biosynthesis Pathway in Cassava Using Comparative Genomic Approach

Autor:	Asawin Meechai, Saowalak Kalapanulak, Supapon Cheevadhanarak, Supatcharee Netrphan, Malinee Suksangpanomrung, Treenut Saithong, Oratai Rongsirikul
Rok vydání:	2010
Předmět:	chemistry.chemical_classification Starch food and beverages Biology biology.organism_classification Genome Metabolic engineering Metabolic pathway chemistry.chemical_compound Enzyme Biochemistry chemistry Amylose Complementary DNA Arabidopsis
Zdroj:	Communications in Computer and Information Science ISBN: 9783642167492 CSBio
Popis:	Cassava is one of the most attractive crops nowadays because it can produce and accumulate large amount of starch in its roots. Cassava starch is widely used as food, feed and raw materials for biochemical industries. Due to the increasing demand of cassava starch, the starch biosynthesis pathway is thus of interest for metabolic engineering, aiming at strain improvement. However, the uncertainties in the metabolic pathway of starch biosynthesis in cassava retard the rate of achievement. Availability of recently released cassava genome motivates us to reconstruct the starch biosynthesis pathway in cassava using comparative genomic approach. Here, nucleotide sequences of the template plants (i.e. Arabidopsis and potato) were compared with the sequence of cassava collected from three sources: Phytozome (genomic sequence), Cassava full-length cDNA and Cassava genome (ESTs) databases. The metabolic pathway of approximately 34 enzymes was constructed, including pathway from sucrose metabolism to amylose and amylopectin synthesis. The resulting pathway is a good initial point toward the complete pathway reconstruction.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::87e57babfdcd8b971f2366c030a6039f https://doi.org/10.1007/978-3-642-16750-8_11 Zobrazit plný text záznamu