Real-Time PCR Detection of the Onion Downy Mildew Pathogen Peronospora destructor From Symptomless Onion Seedlings and Soils
| Autor: | Atsushi Miyasaka, Nobuya Tashiro, Yutaka Iwamoto, Motoaki Kusaba, Kazuki Fujiwara, Hiroyoshi Inoue, Ryoichi Sonoda |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
0106 biological sciences
0301 basic medicine Peronospora destructor Outbreak Plant Science Biology biology.organism_classification 01 natural sciences Conidium Fungicide 03 medical and health sciences Horticulture 030104 developmental biology Downy mildew Oospore Destructor Agronomy and Crop Science Pathogen 010606 plant biology & botany |
| Zdroj: | Plant Disease. 105:643-649 |
| ISSN: | 1943-7692 0191-2917 |
| DOI: | 10.1094/pdis-05-20-1095-re |
| Popis: | An outbreak of downy mildew disease of onion, caused by Peronospora destructor, in Japan in 2016 necessitated a reevaluation of the primary inoculum sources to optimize disease management. Detection of the P. destructor pathogen in plants with asymptomatic infection and in soil would guide the application of fungicides according to the extent of infection before disease development. Here, we detected P. destructor in both plants and soil using newly developed primer sets (Pd ITS and Pd ITS 614) by both conventional and real-time PCR. Validation by real-time PCR with Pd ITS 614 showed that P. destructor DNA was amplified from symptomless seedlings at 3.7 × 102 to 1.0 × 100 conidium cells/50 mg leaf tissue, suggesting the detection of asymptomatic infection. Real-time PCR with Pd ITS amplified pathogen DNA from field soils at 1.6 × 103 to 8.3 × 101 oospore cells/g of soil. This real-time PCR assay provides a useful tool for identifying and quantifying inoculum sources, which may be the foundation of the design of integrated disease management strategies. |
| Databáze: | OpenAIRE |
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