Ilex paraguariensis extract provides increased resistance against oxidative stress and protection against Amyloid beta-induced toxicity compared to caffeine in Caenorhabditis elegans
| Autor: | Leticia Priscilla Arantes, Fabiane Baptista Bicca Obetine, Tássia Limana da Silveira, Aline Franzen da Silva, Marina Lopes Machado, Larissa Marafiga Cordeiro, Riva de Paula Oliveira, Félix Alexandre Antunes Soares, Ivana Beatrice Mânica da Cruz, Daniele Coradini Zamberlan |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Antioxidant Amyloid beta medicine.medical_treatment Medicine (miscellaneous) Pharmacology medicine.disease_cause Neuroprotection 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine medicine chemistry.chemical_classification Reactive oxygen species 030109 nutrition & dietetics Nutrition and Dietetics biology General Neuroscience General Medicine Heat shock factor chemistry Toxicity biology.protein Caffeine 030217 neurology & neurosurgery Oxidative stress |
| Zdroj: | Nutritional Neuroscience. 24:697-709 |
| ISSN: | 1476-8305 1028-415X |
| Popis: | Ilex paraguariensis is a plant from South America, used to prepare a tea-like beverage rich in caffeine and polyphenols with antioxidant proprieties. Caffeine consumption is associated with a lower risk of age-associated neuropathologies, besides several extracts that have antioxidant proprieties are known to be neuroprotective, and oxidative stress strongly correlates with Aβ-toxicity. This study aims to investigate the neuroprotective effects of the Ilex paraguariensis hydroalcoholic extract (IPHE) and to evaluate if caffeine agent present in IPHE exerts neuroprotective effects in an amyloid beta-peptide (Aβ)-induced toxicity in Caenorhabditis elegans. The wild-type and CL2006 worms were treated with IPHE (2 and 4 mg/mL) or caffeine (200 and 400 μM) since larval stage 1 (L1) until they achieved the required age for each assay. IPHE and caffeine increased the lifespan and appeared to act directly by reactive oxygen species (ROS) scavenger in both wild-type and CL2006 worms, also conferred resistance against oxidative stress in wild-type animals. Furthermore, both treatments delayed Aβ-induced paralysis and decreased AChE activity in CL2006. The protective effect of IPHE against Aβ-induced paralysis was found to be dependent on heat shock factor hsf-1 and FOXO-family transcription factor daf-16, which are respectively involved in aging-related processes and chaperone synthesis, while that of caffeine was dependent only on daf-16. Mechanistically, IPHE and caffeine decreased the levels of Aβ mRNA in the CL2006 worms; however, only IPHE induced expression of the heat shock chaperonin hsp-16.2, involved in protein homeostasis. The results were overall better when treated with IPHE than with caffeine. |
| Databáze: | OpenAIRE |
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