Principles and applications of matrix-assisted UV-laser desorption/ionization mass spectrometry
| Autor: | Arndt Ingendoh, Michael Karas, Eckhard Nordhoff, Kerstin Strupat, U. Bahr, Franz Hillenkamp, Bernd Stahl |
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| Rok vydání: | 1990 |
| Předmět: |
chemistry.chemical_classification
Chromatography Protein mass spectrometry Chemistry Biomolecule Analytical chemistry Mass spectrometry Biochemistry Analytical Chemistry Surface-enhanced laser desorption/ionization Ion Matrix (chemical analysis) Membrane Desorption Environmental Chemistry Spectroscopy |
| Zdroj: | Analytica Chimica Acta. 241:175-185 |
| ISSN: | 0003-2670 |
| DOI: | 10.1016/s0003-2670(00)83645-4 |
| Popis: | Matrix-assisted laser desorption/ionization mass spectrometry (LDI MS) has shown its potential for desorbing ions of biomolecules with relative molecular masses ( M r ) up to about 300 000 daltons. Determinations of M r with an accuracy of 0.01–0.2% from about 1 pmol of sample are possible. Examples are shown for proteins, glycoproteins, oligonucleotides and carbohydrates. Examples of the combination of LDI MS with biochemical methods are shown, including the determination of the carbohydrate content of glycoproteins, the identification of disulphide-bonded subunits by cleavage of the light and heavy chains of a monoclonal antibody, the detection of the time course of enzymatic reactions and the analysis of proteins bound to a PVDF membrane. |
| Databáze: | OpenAIRE |
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