Antioxidant activity and phenolic compounds of buckwheat and barley by the data of spectrophotometry and HPLC
| Autor: | S. S. Aleksenko |
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| Rok vydání: | 2013 |
| Předmět: | |
| Zdroj: | Journal of Analytical Chemistry. 68:458-465 |
| ISSN: | 1608-3199 1061-9348 |
| Popis: | The antioxidant activity of buckwheat and barley extracts by reaction with 1,1-diphenyl-2-picrylhydrazyl and the total of phenolic compounds have been determined using the Folin-Ciocalteu reagent. It has been found that water-ethanol extracts of buckwheat are characterized by higher antioxidant activity (6.2 ± 0.5 μM-eq. of Trolox/g) and concentration of phenolic compounds (4.41 ± 0.07 mg-eq. of rutin/g) compared to barley extracts (4.2 ± 0.3 μM-eq. of Trolox/g and 2.4 ± 0.1 mg-eq. of rutin/g, respectively). A series of phenolic compounds have been identified by HPLC with UV detection and mass spectrometric detection with electrospray ionization. The main phenolic compounds-antioxidants in buckwheat extracts are rutin, catechin and epicatechin, 1-O-caffeoyl-O-rutinoside (m/z 487), and epicatechin-O-3,4-dimethylgallate (m/z 469), and in the barley extract, catechin, prodelphinidin B3 (m/z 593), procyanidin B3 (m/z 577), and procyanidin C2 (m/z 865). |
| Databáze: | OpenAIRE |
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