Visualization of Scalar Distribution in Microchannel Using 2-Color LIF

Autor:	Takashi Saito, Teruo Nishida, Hiroyuki Akimoto, Souichi Saeki, Kazuyuki Nakamura, Motoya Kaneta
Rok vydání:	2005
Předmět:	Physics Light intensity Optics Microchannel business.industry Scalar (mathematics) Calibration business Scalar field Excitation Intensity (heat transfer) Linear function
Zdroj:	Journal of the Visualization Society of Japan. 25:301-304
ISSN:	1884-037X 0916-4731
Popis:	This paper presents a novel scalar field measurement for microfluidic devices; "2-Color Laser-induced Fluorescence (2-Color LIF)". In general, a conventional method (1-Color LIF) strongly depends on the excitation intensity distribution, which is generated by spatio-temporal intensity fluctuation of light itself and configuration of microchannel. In contrast, the proposed method can eliminate the undesirable influence using a ratio-metric technique with two fluorescence images, because fluorescence intensity can be approximately a linear function of the excitation light intensity. Therefore, it is possible to measure the scalar distribution quantitatively and simplify its calibration. In this experiment, this was applied to calibrate between either temperature or pH and normalized ratio value by dividision between two fluorescence images. Consequently, spatial intensity error can be reduced to about 95% as compared with 1-Color LIF image. The scalar distribution in microchannel was visualized with high resolution 0.24um×0.24μm.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::8522f25076c0b1ec8abec4fafb2b4b0e https://doi.org/10.3154/jvs.25.supplement1_301 Zobrazit plný text záznamu