Nanotechnological innovation for the production of daughter less Tilapia, Oreochromis niloticus (Linnaeus, 1758)
| Autor: | Rupam Sharma, Upasana Sahoo, Subodh Gupta, W. S. Lakra, Harshavardhan D. Joshi, V. K. Tiwari |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
food.ingredient
Dose 02 engineering and technology 030226 pharmacology & pharmacy General Biochemistry Genetics and Molecular Biology 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine food Animal science medicine Methyltestosterone Testosterone General Environmental Science General Immunology and Microbiology biology Tilapia 021001 nanoscience & nanotechnology biology.organism_classification PLGA Oreochromis Fadrozole chemistry Drug delivery 0210 nano-technology General Agricultural and Biological Sciences medicine.drug |
| Zdroj: | Journal of Applied and Natural Science. 9:1921-1925 |
| ISSN: | 2231-5209 0974-9411 |
| DOI: | 10.31018/jans.v9i4.1464 |
| Popis: | The aim of present work was to develop a new Fadrozole (FDZ)-loaded Poly (D,L-lactide-co– glycolide) lactide:glycolide (50:50)(PLGA) nanoparticles for effective delivery of the masculinization drug, Fadrozole, as an alternative to commercially available masculinization agents like testosterone (dietary supplementation of 17 α- methyltestosterone) which are steroids and banned in most EU countries. The FDZ-loaded PLGA NPs were pre-pared by solvent displacement technique. The particle size of FDZ-loaded PLGA NPs was analyzed using LICOMP particle size analyser. It was found to be in the range of 60±66.7 nm to 560±66.7 nm with average size of 201.4±66.7 nm, where the Zeta potential was estimated to be about -20.82 mV, a series of experiments were carried out to induce masculinization using FDZ-loaded PLGA nanoparticles during the sex differentiation period. Tilapia, Oreochromis niloticus fry were treated with FTZ-loaded PLGA nanoparticles at dosages 5, 25, 50 and 100ppm/kg diet for 10, 15 and 30 days. The results indicated an increase in the proportion of males with dosage and duration of treatment. The male percentage was 92.35±0.86 for T7(50 ppm) at 10 days, 97.76±1.12 for T7 (100 ppm) at 15 days and 100 % for both T6 (50ppm) and T7 (100 ppm) at 30 days. This is first time done by using nanotechnology efficiently in Tilapia species which is very important Fresh water aquaculture species in present era. Which showed increase the male population with lesser dose of nano-encapsulated Fadrozole (FDZ) loaded PLGA nanoparticles drug as compared with naked control Fadrozole (FDZ) drug delivery. |
| Databáze: | OpenAIRE |
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