Development of volume-stable adipose tissue constructs using polycaprolactone-based polyurethane scaffolds and fibrin hydrogels
| Autor: | Katharina Wittmann, Torsten Blunk, Helena Mayer, Katharina Storck, Rainer Staudenmaier, Christian Muhr, Petra Bauer-Kreisel, Gerhard Maier, Hinrich Wiese, Sybille Regn |
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| Rok vydání: | 2013 |
| Předmět: |
0301 basic medicine
Scaffold biology Biomedical Engineering Medicine (miscellaneous) Adipose tissue 02 engineering and technology 021001 nanoscience & nanotechnology Fibrin Biomaterials 03 medical and health sciences chemistry.chemical_compound 030104 developmental biology chemistry Tissue engineering Adipogenesis In vivo Polycaprolactone Self-healing hydrogels biology.protein 0210 nano-technology Biomedical engineering |
| Zdroj: | Journal of Tissue Engineering and Regenerative Medicine. 10:E409-E418 |
| ISSN: | 1932-6254 |
| DOI: | 10.1002/term.1830 |
| Popis: | Adipose tissue engineering aims at the restoration of soft tissue defects and the correction of contour deformities. It is therefore crucial to provide functional adipose tissue implants with appropriate volume stability. Here, we investigate two different fibrin formulations, alone or in combination with biodegradable polyurethane (PU) scaffolds as additional support structures, with regard to their suitability to generate volume-stable adipose tissue constructs. Human adipose-derived stem cells (ASCs) were incorporated in a commercially available fibrin sealant as well as a stable fibrin hydrogel previously developed by our group. The composite constructs made from the commercially available fibrin and porous poly(e-caprolactone)-based polyurethane scaffolds exhibited increased volume stability as compared to fibrin gels alone; however, only constructs using the stable fibrin gels completely maintained their size and weight for 21 days. Adipogenesis of ASCs was not impaired by the additional PU scaffold. After induction with a common hormonal cocktail, for constructs with either fibrin formulation, strong adipogenic differentiation of ASCs was observed after 21 days in vitro. Furthermore, upregulation of adipogenic marker genes was demonstrated at mRNA (PPARγ, C/EBPα, GLUT4 and aP2; qRT–PCR) and protein (leptin; ELISA) levels. Stable fibrin/PU constructs were further evaluated in a pilot in vivo study, resulting in areas of well-vascularized adipose tissue within the implants after only 5 weeks. Copyright © 2013 John Wiley & Sons, Ltd. |
| Databáze: | OpenAIRE |
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