Immunohistochemical Diagnosis Method in Parvovirus
| Autor: | Marius Pente, Alina Ghise, J. Dégi, Mihaela Petcu, Adrian Stancu, Sorin Aurelian Pasca, Romeo Teodor Cristina, Delia Mira Berceanu-Vaduva, Liliana Carpinisan, Camelia Tulcan, Dana Emilia Velimirovici |
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| Rok vydání: | 2020 |
| Předmět: |
Pathology
medicine.medical_specialty biology medicine.drug_class Parvovirus Materials Science (miscellaneous) Process Chemistry and Technology Crypt General Engineering General Chemistry General Medicine Disease Clostridium perfringens Monoclonal antibody biology.organism_classification medicine.disease_cause General Biochemistry Genetics and Molecular Biology Antigen Materials Chemistry Etiology medicine Immunohistochemistry General Pharmacology Toxicology and Pharmaceutics |
| Zdroj: | Revista de Chimie. 71:491-496 |
| ISSN: | 2668-8212 0034-7752 |
| DOI: | 10.37358/rc.20.7.8268 |
| Popis: | 10 cases of histopathologically suggestive lesions for parvovirus in the canine species were examined by the avidin-biotin-peroxidase complex For this, a mouse monoclonal antibody was used Following the immunohistochemical examination, positive results were obtained in 80% (8 dogs) of processed samples The most frequent positive immunohistochemical reaction was observed in macrophages, lymphocytes, necrotic cells of the intestinal crypt, evidence of parvoviral antigen is reliable for determining the etiology of the disease if parvovirus is suspected on the basis of the lesions In order to perform the diagnostic immunohistochemical procedure, the strepto-avidin-biotin-peroxidase method was used, using the monoclonal antibody It is of murine origin Negative cases of parvovirus by immunohistochemical examination show the existence of other causes that produce the same lesions;coronaviruses, rotaviruses or coronaviruses associated with clostridium perfringens which may cause intestinal necrosis of the crypt All dog breeds can do this disease, but in the Poodle breed the disease has been more common Sex is not a criterion of receptivity, but in proportion of over 60% were affected females Regarding the seasonal evolution, the disease is more common in the winter period compared to the other seasons © 2020 SYSCOM 18 S R L All rights reserved |
| Databáze: | OpenAIRE |
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