Detection and recovery ofLegionella in water

Autor:	W. E. Morrill, J. M. Barbaree, W. T. Martin, G. N. Sanden, B. S. Fields
Rok vydání:	1988
Předmět:	Chromatography biology Legionella Health Toxicology and Mutagenesis Toxicology biology.organism_classification Stain Legionella pneumophila Water sample Microbiology law.invention Environmental water law Acid treatment Direct fluorescent antibody Filtration Water Science and Technology
Zdroj:	Toxicity Assessment. 3:479-490
ISSN:	1098-2256 0884-8181
Popis:	Methods used to detect Legionella include direct fluorescent antibody stain, genetic probe, and culturing. Culturing is preferred for detecting Legionella pneumophila (Lp) in water; however, recovery rates are affected by treatment of water samples. Experiments with water-containing Lp showed that (a) a water storage temperature of 4°C results in a slower decline in numbers of Lp as compared with 25, 35, and 45°C; (b) a water sample containing Lp and nonlegionellae microflora is appropriate for quality control of selective media used to isolate Legionella from environmental water specimens; and (c) acid treatment and concentration of water by filtration are effective techniques for isolating Lp from environmental water samples, but they do not yield 100% recovery of Lp cells.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::83e864954b6d701828cb73be5f3015d4 https://doi.org/10.1002/tox.2540030505 Zobrazit plný text záznamu Plný text