Detection and recovery ofLegionella in water
Autor: | W. E. Morrill, J. M. Barbaree, W. T. Martin, G. N. Sanden, B. S. Fields |
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Rok vydání: | 1988 |
Předmět: | |
Zdroj: | Toxicity Assessment. 3:479-490 |
ISSN: | 1098-2256 0884-8181 |
Popis: | Methods used to detect Legionella include direct fluorescent antibody stain, genetic probe, and culturing. Culturing is preferred for detecting Legionella pneumophila (Lp) in water; however, recovery rates are affected by treatment of water samples. Experiments with water-containing Lp showed that (a) a water storage temperature of 4°C results in a slower decline in numbers of Lp as compared with 25, 35, and 45°C; (b) a water sample containing Lp and nonlegionellae microflora is appropriate for quality control of selective media used to isolate Legionella from environmental water specimens; and (c) acid treatment and concentration of water by filtration are effective techniques for isolating Lp from environmental water samples, but they do not yield 100% recovery of Lp cells. |
Databáze: | OpenAIRE |
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