Genetic diversity of the endangered endemic species Hedysarum sangilense Krasnoborov et Timokhina (Fabaceae)

Autor:	Elena S. Konichenko, Inessa Yu. Selyutina, Evgeniy G. Zibzeev, Irina N. Kuban
Rok vydání:	2021
Předmět:	Genetic diversity Ecology Hedysarum Endangered species Plant Science Fabaceae Biology Endemism biology.organism_classification Ecology Evolution Behavior and Systematics
Zdroj:	Botanica Pacifica. 10
ISSN:	2410-3713 2226-4701
DOI:	10.17581/bp.2021.10109
Popis:	Hedysarum sangilense Krasnoborov et Timokhina (Fabaceae) is а rare subendemic species of the Fabaceae family. It has been found in a restricted area, only in isolated habitats in Tyva Republic and Northern Mongolia. Two PCR techniques, using RAPD and inter-simple sequence repeat (ISSR) markers, were used to perform a comparative analysis of genetic diversity in this species. When amplifying DNA with three RAPD primers, we produced 51 bands, of which 21 (40.6 %) were polymorphic. Amplification of genomic DNA using ISSR analysis yielded 96 fragments, of which 35 (36.6 %) were polymorphic. Nei,s gene diversity (H) was estimated to be 0.105 within populations (range 0.086–0.150) and 0.191 at the species level. Genetic differentiation among populations (GST) was 0.383. The results indicate that both of the marker systems RAPD and ISSR, individually or in combination, can be effectively used in the determination of the genetic relationship among and within populations of H. sangilense.
Databáze:	OpenAIRE
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