Photoswitchable fluorescent polymeric nanoparticles for rewritable fluorescence patterning and intracellular dual-color imaging with AIE-based fluorogens as FRET donors
| Autor: | Zhong Weibang, Jian Chen, Peisheng Zhang, Xuyao Zeng, Hong Yongxiang, Lehui Xiao |
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| Rok vydání: | 2017 |
| Předmět: |
Materials science
Polymers and Plastics Photoisomerization Organic Chemistry Nanoparticle Bioengineering Nanotechnology 02 engineering and technology 010402 general chemistry 021001 nanoscience & nanotechnology Photochemistry 01 natural sciences Biochemistry Fluorescence 0104 chemical sciences Photochromism Förster resonance energy transfer Irradiation 0210 nano-technology Intracellular Visible spectrum |
| Zdroj: | Polymer Chemistry. 8:4849-4855 |
| ISSN: | 1759-9962 1759-9954 |
| DOI: | 10.1039/c7py00834a |
| Popis: | Photoswitchable fluorescent polymer nanoparticles (PFPNs) were constructed from an aggregation-induced emission (AIE) based fluorogen and a photochromic spiropyran-linked amphiphilic copolymer via a simple co-precipitation method. Possessing the reversible photoisomerization property and high fluorescence resonance energy transfer (FRET) efficiency, not only did the designed spiropyran-linked amphiphilic copolymer provide the PFPNs with excellent long-term stability (∼5 weeks) in aqueous media, but also it could both efficiently and reversibly switch on/off the fluorescence emission of the AIE-based fluorogen upon irradiation with UV and visible light. It is worth noting that the high contrast dual-color characteristics written with PFPNs as rewritable fluorescence patterning can be tuned by altering the wavelength of the irradiation light. Moreover, the resultant PFPNs were successfully applied for intracellular dual-color imaging due to their outstanding cell-membrane permeability and rapid photo-responsiveness, as well as excellent repeatability properties. |
| Databáze: | OpenAIRE |
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