A Two-Photon Fluorescent Probe for Imaging Endogenous ONOO– near NMDA Receptors in Neuronal Cells and Hippocampal Tissues
| Autor: | Dayoung Lee, Juyoung Yoon, Hwan Myung Kim, Chang Su Lim, Myoung Ki Cho, Gyeongju Ko, Sang Jip Nam, Dayoung Kim |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
inorganic chemicals
Chemistry Endogeny 02 engineering and technology Hippocampal formation 010402 general chemistry 021001 nanoscience & nanotechnology 01 natural sciences Fluorescence 0104 chemical sciences Analytical Chemistry chemistry.chemical_compound Two-photon excitation microscopy Fluorescence microscope Biophysics NMDA receptor 0210 nano-technology Receptor Peroxynitrite |
| Zdroj: | Analytical Chemistry. 90:9347-9352 |
| ISSN: | 1520-6882 0003-2700 |
| Popis: | In this study, we developed a two-photon fluorescent probe for detection of peroxynitrite (ONOO–) near the N-methyl-d-aspartate (NMDA) receptor. This naphthalimide-based probe contains a boronic acid reactive group and an ifenprodil-like tail, which serves as an NMDA receptor targeting unit. The probe displays high sensitivity and selectivity, along with a fast response time in aqueous solution. More importantly, the probe can be employed along with two-photon fluorescence microscopy to detect endogenous ONOO– near NMDA receptors in neuronal cells as well as in hippocampal tissues. The results suggest that the probe has the potential of serving as a useful imaging tool for studying ONOO– related diseases in the nervous system. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|