Additional file 2 of ADAM22/LGI1 complex as a new actionable target for breast cancer brain metastasis

Autor: Charmsaz, Sara, Doherty, Ben, Cocchiglia, Sinéad, Varešlija, Damir, Marino, Attilio, Cosgrove, Nicola, Marques, Ricardo, Priedigkeit, Nolan, Purcell, Siobhan, Bane, Fiona, Jarlath Bolger, Byrne, Christopher, O’Halloran, Philip J., Brett, Francesca, Sheehan, Katherine, Brennan, Kieran, Hopkins, Ann M., Keelan, Stephen, Jagust, Petra, Madden, Stephen, Martinelli, Chiara, Battaglini, Matteo, Oesterreich, Steffi, Lee, Adrian V., Ciofani, Gianni, Hill, Arnold D. K., Young, Leonie S.
Předmět:
Popis: Additional file 2: Figure S1. Confirmation of LY2 ADAM22 CRISPR knockout cell line. (a) RFP expression confirming CRISPR /Cas9 mediated double stranded break and insertion of an RFP tagged HDR plasmid (b) FACS gating strategy based on high RFP expression to single cell sort CRISPR/Cas9 ADAM22 KO cells (right) from LY2 WT cells (left) (c) Confirmation of ADAM22 gene silencing in an LY2 ADAM22 KO clone (Clone H (C-H)) by PCR and Western blot analysis (n = 4). *** p
Databáze: OpenAIRE