Diagnosis of Phlebotomas Argentipes as a Vector for Visceral Leismaniasis by PCR in Bangladesh

Autor:	Sma Shamsuzzaman, SM Shamsuzzaman, Jalal Uddin Asharful Haq, Farida Huq, Zaforullah Chowdhury
Rok vydání:	2018
Předmět:	0301 basic medicine 03 medical and health sciences 030104 developmental biology Vector (epidemiology) parasitic diseases Parasite hosting Buffy coat Biology biology.organism_classification Virology DNA extraction Sandfly
Zdroj:	Update Dental College Journal. 7:15-18
ISSN:	2307-3160 2226-8715 2001-2004
DOI:	10.3329/updcj.v7i2.36208
Popis:	Objectives: The present study was undertaken to diagnose sandfly as a vector of visceral leismaniasis by PCR in Bangladesh. Place and period of study: The study was conducted in Fulbaria Upazilla of Mymensing District during 2001-2004.Materials & Methods: The study was conducted in the department of Microbiology, National Institude of Preventive and social medicine (NIPSOM), Mohakhali, Dhaka. DNA extraction from Sand Fly: All the procedure followed for DNA extraction from Bone marrow is same for sandfly except AL buffer where instead of AL buffer ATL buffer were added. The primers used are constructed from kDNA of L. (L) donovani. DD8 strain to amplify a fragment of 354 bp in length.Results: PCR of extracted DNA from sandfly (P.argentipes) revealed 354 bp bands similar to buffy coat and bone mamow samples containing DNA of L. Donovani. This might be the first demonstration of L.donovani parasite in sand fly vector in Bangladesh.Conclusion: The present study shows that PCR is a good diagnostic tool for the demonstration of L.donovani parasite for the P.argentips sp in Bangladesh.Update Dent. Coll. j: 2017; 7 (2): 15-18
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::826aa6f3cbab452dfcb373f4c2cf2234 https://doi.org/10.3329/updcj.v7i2.36208 Zobrazit plný text záznamu