Interaction with calf-thymus DNA and photoinduced cleavage of pBR322 by rhodium(III) and iridium(III) complexes containing crown thioether ligands
| Autor: | Jisook Kim, Gregory J. Grant, Hitesh M. Vashi, Ashley D. Cardenal, Titus V. Albu, Weinan Chen, Hendrik J. Greve |
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| Rok vydání: | 2018 |
| Předmět: |
010405 organic chemistry
Stereochemistry chemistry.chemical_element 010402 general chemistry Cleavage (embryo) 01 natural sciences Medicinal chemistry Fluorescence 0104 chemical sciences Rhodium Inorganic Chemistry Metal chemistry.chemical_compound chemistry Thioether visual_art Materials Chemistry visual_art.visual_art_medium Iridium Physical and Theoretical Chemistry Ethidium bromide DNA |
| Zdroj: | Inorganica Chimica Acta. 469:484-494 |
| ISSN: | 0020-1693 |
| DOI: | 10.1016/j.ica.2017.10.005 |
| Popis: | In this report, we present our investigation on the photoinduced cleavage of plasmid pBR322 and the binding interactions with calf-thymus (CT) DNA by a series of thioether metal complexes. The complexes of interest are rhodium and iridium complexes containing thiacrown ligands 1,4,7-trithiacyclononane (9S3) and 1-oxa-4,7-dithiacyclononane (9S2O), and the complexes are abbreviated as [Rh(9S3)Cl3], [Rh(9S2O)Cl3], and [Ir(9S3)Cl3]. In the nicking assay, pBR322 was treated with each complex and irradiated at 254 and 350 nm, respectively, in concentration- and time-dependent studies. The nicking assay revealed that, under exposure to 254-nm radiation, [Rh(9S3)Cl3] and [Rh(9S2O)Cl3] cleaved pBR322 efficiently forming a nicked form, while [Ir(9S3)Cl3] was least efficient. For the 350-nm irradiation, a similar trend was observed, with [Rh(9S3)Cl3] being the most efficient one, however with a lower efficiency than at 254 nm. An ethidium bromide displacement assay was also carried out to evaluate the binding interaction of each compound with CT-DNA by titrating the pre-equilibrated complex of CT-DNA and EB with the investigated complexes. The efficient concentration to achieve a 50% loss in fluorescent emission was found to be 24 μM for [Rh(9S3)Cl3] and 35 μM for [Rh(9S2O)Cl3], while [Ir(9S3)Cl3] was an ineffective DNA binder. |
| Databáze: | OpenAIRE |
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