Euglena gracilis cadmium-binding protein-II contains sulfide ion

Autor:	David H. Petering, D N Weber, C F Shaw rd
Rok vydání:	1987
Předmět:	chemistry.chemical_classification Euglena gracilis Sulfide ved/biology DTNB Chemistry Dithionitrobenzoic Acid Inorganic chemistry ved/biology.organism_classification_rank.species Cell Biology Biochemistry chemistry.chemical_compound Reagent Thiol Molecular Biology Methylene blue Stoichiometry
Zdroj:	Journal of Biological Chemistry. 262:6962-6964
ISSN:	0021-9258
DOI:	10.1016/s0021-9258(18)48186-0
Popis:	Sulfide ions are a constituent of the cadmium-binding protein-II in the alga Euglena gracilis. Their presence was demonstrated by the methylene blue assay, by acid labilization induced reductions in the Cd-S charge transfer band at 254 nm and by reactions with the thiol reagent, 5,5'-dithiobis-(2-nitrobenzoic acid) (DTNB). Direct reduction of DTNB by sulfide and precipitation of CdS yield a complex stoichiometry for the DTNB reaction. The S2-/Cd2+ ratios determined, 1.25 +/- 0.10 (methylene blue) and 1.37 +/- 0.16 (DTNB), are in good agreement.
Databáze:	OpenAIRE
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