Protein restriction during peripubertal period impairs endothelial aortic function in adult male Wistar rats

Autor: Amanda Cristina de Souza, Deborah Gomes da Silva, Juliana da Silva Jezuíno, Anna Rebeka Oliveira Ferreira, Maiara Vanusa Guedes Ribeiro, Camila Borecki Vidigal, Kawane Fabricio Moura, Rafaela Pires Erthal, Paulo Cezar de Freitas Mathias, Glaura Scantamburlo Alves Fernandes, Kesia Palma-Rigo, Graziela Scalianti Ceravolo
Rok vydání: 2023
Předmět:
Zdroj: Journal of Developmental Origins of Health and Disease. :1-8
ISSN: 2040-1752
2040-1744
Popis: Protein restriction during early phases of body development, such as intrauterine life can favor the development of vascular disorders. However, it is not known if peripubertal protein restriction can favor vascular dysfunction in adulthood. The present study aimed to evaluated whether a protein restriction diet during peripubertal period favors endothelial dysfunction in adulthood. Male Wistar rats from postnatal day (PND) 30 until 60 received a diet with either 23% protein (CTR group) or with 4% protein (LP group). At PND 120, the thoracic aorta reactivity to phenylephrine, acetylcholine, and sodium nitroprusside was evaluated in the presence or absence of: endothelium, indomethacin, apocynin and tempol. The maximum response (Rmax) and pD2 (-log of the concentration of the drug that causes 50% of the Rmax) were calculated. The lipid peroxidation and catalase activity were also evaluated in the aorta. The data were analyzed by ANOVA (one or two-ways and Tukey’s) or independent t-test; the results were expressed as mean ± S.E.M., p < 0.05. The Rmax to phenylephrine in aortic rings with endothelium were increased in LP rats when compared with the Rmax in CTR rats. Apocynin and tempol reduced Rmax to phenylephrine in LP aortic rings but not in CTR. The aortic response to the vasodilators was similar between the groups. Aortic catalase activity was lower and lipid peroxidation was greater in LP compared to CTR rats. Therefore, protein restriction during the peripubertal period causes endothelial dysfunction in adulthood through a mechanism related to oxidative stress.
Databáze: OpenAIRE
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