Cryptogein, a fungal elicitor, remodels the phenylpropanoid metabolism of tobacco cell suspension cultures in a calcium-dependent manner
| Autor: | Jacques Haiech, Christian Brière, Audrey Carrouche, Jacqueline Grima-Pettenati, Nicolas Amelot, Christian Mazars, Alain Pugin, Raoul Ranjeva, Stéphane Bourque, Saïda Danoun |
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| Rok vydání: | 2010 |
| Předmět: |
0106 biological sciences
Calcium metabolism 0303 health sciences Fungal protein biology Phenylpropanoid Physiology Aequorin chemistry.chemical_element Plant Science Calcium 01 natural sciences Elicitor 03 medical and health sciences chemistry Biochemistry Transcriptional regulation biology.protein 030304 developmental biology 010606 plant biology & botany Calcium signaling |
| Zdroj: | Plant, Cell & Environment. 34:149-161 |
| ISSN: | 0140-7791 |
| Popis: | Plant cells use calcium-based signalling pathways to transduce biotic and/or abiotic stimuli into adaptive responses. However, little is known about the coupling between calcium signalling, transcriptional regulation and the downstream biochemical processes. To understand these relationships better, we challenged tobacco BY-2 cells with cryptogein and evaluated how calcium transients (monitored through the calcium sensor aequorin) impact (1) transcript levels of phenylpropanoid genes (assessed by RT-qPCR); and (2) derived-phenolic compounds (analysed by mass spectrometry). Most genes of the phenylpropanoid pathway were up-regulated by cryptogein and cell wall-bound phenolic compounds accumulated (mainly 5-hydroxyferulic acid). The accumulation of both transcripts and phenolics was calcium-dependent. The transcriptional regulation of phenylpropanoid genes was correlated in a non-linear manner with stimulus intensity and with components of the cryptogein-induced calcium signature. In addition, calmodulin inhibitors increased the sensitivity of cells to low concentrations of cryptogein. These results led us to propose a model of coupling between the cryptogein signal, calcium signalling and the transcriptional response, exerting control of transcription through the coordinated action of two decoding modules exerting opposite effects. |
| Databáze: | OpenAIRE |
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